Replication Stress and Aging in Budding Yeast
Author Information
Author(s): Weinberger Martin, Feng Li, Paul Anita, Smith Daniel L. Jr., Hontz Robert D., Smith Jeffrey S., Vujcic Marija, Singh Keshav K., Huberman Joel A., Burhans William C.
Primary Institution: Roswell Park Cancer Institute
Hypothesis
Inhibition of nutrient-signaling pathways can extend the chronological lifespan of budding yeast by inducing a more efficient nutrient depletion-induced G1 arrest.
Conclusion
Replication stress is an important determinant of chronological lifespan in budding yeast, and conditions that promote a more efficient G1 arrest can extend lifespan.
Supporting Evidence
- Caloric restriction and osmotic stress extend chronological lifespan in yeast.
- Deletion of SCH9 enhances Rim15-dependent stress responses and extends chronological lifespan.
- Nutrient depletion induces DNA replication stress, which is mitigated by Mec1 and Rad53.
Takeaway
When yeast cells run out of food, they can stop growing to survive longer. If they don't stop growing properly, it can hurt their lifespan.
Methodology
The study measured the frequency of G1 arrest and apoptosis in yeast cells during nutrient depletion under various conditions.
Digital Object Identifier (DOI)
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