Genetic Characterization of Japanese Encephalitis Virus Isolates Circulating in Mosquitoes from Pig and Sheep Farms in Shanghai, China
2024

Study of Japanese Encephalitis Virus in Mosquitoes in Shanghai

Sample size: 24073 publication 10 minutes Evidence: moderate

Author Information

Author(s): Zhang Hailong, Li Dan, Zheng Jiayang, Zhang Yan, Li Zongjie, Liu Ke, Li Beibei, Qiu Yafeng, Shao Donghua, Wai Soesoe, Wei Jianchao, Ma Zhiyong, Liu Juxiang, Liu Huisheng, Liu Kaituo

Primary Institution: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences

Hypothesis

What is the prevalence of Japanese encephalitis virus genotypes in mosquitoes from pig and sheep farms in Shanghai?

Conclusion

The study found that genotype I and genotype III of the Japanese encephalitis virus are currently circulating in mosquitoes in Shanghai, with a significant presence in Culex tritaeniorhynchus.

Supporting Evidence

  • 24,073 mosquitoes were collected from pig and sheep farms in Shanghai.
  • Culex tritaeniorhynchus was the most common species found.
  • Six strains of Japanese encephalitis virus were isolated, with five classified as genotype I and one as genotype III.
  • This study reports the first isolation of a genotype I strain from mosquitoes in sheep farms.
  • The minimum infection rate of JEV was calculated to be 0.8 per 1000 mosquitoes.

Takeaway

Researchers collected a lot of mosquitoes to see if they carried a virus that can make people sick, and they found some that did.

Methodology

Mosquitoes were collected using UV light traps, sorted into pools, and tested for the Japanese encephalitis virus using PCR.

Limitations

The study was limited to specific districts in Shanghai and may not represent the entire region.

Digital Object Identifier (DOI)

10.3390/ani14243653

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