The Interaction of the Antitumor Complexes Na[trans-RuCl4(DMSO)(Im)] and Na[trans-RuCl4(DMSO)(Ind)] with Apotransferrin: A Spectroscopic Study
1996
Interaction of Antitumor Complexes with Apotransferrin
publication
Evidence: moderate
Author Information
Author(s): Luigi Messori, Felix Kratz, Enzo Alessio
Primary Institution: Department of Chemistry, University of Florence
Hypothesis
The study investigates how two antitumor ruthenium(III) complexes interact with human serum apotransferrin.
Conclusion
The study demonstrates that the ruthenium(III) complexes form stable adducts with apotransferrin, which can be detached under acidic conditions.
Supporting Evidence
- The complexes hydrolyze in a physiological buffer, and their interaction with apotransferrin enhances this process.
- CD spectroscopy confirmed the formation of specific adducts between the complexes and apotransferrin.
- 1H NMR spectroscopy showed changes in the spectra indicating binding of hydrolysis products to apotransferrin.
Takeaway
The researchers looked at how certain metal compounds used in cancer treatment interact with a protein in the blood, finding that they can stick together and be released later.
Methodology
The interaction was studied using UV-Vis absorption, CD, and 1H NMR spectroscopy.
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