Pore dilation occurs in TRPA1 but not in TRPM8 channels
2009
Pore Dilation in TRPA1 Channels
publication
Evidence: moderate
Author Information
Author(s): Chen Jun, Kim Donghee, Bianchi Bruce R, Cavanaugh Eric J, Faltynek Connie R, Kym Philip R, Reilly Regina M
Primary Institution: Neuroscience, Global Pharmaceutical Research and Development, Abbott Laboratories
Hypothesis
It is unknown whether TRPA1 and TRPM8 undergo pore dilation.
Conclusion
The study shows that pore dilation occurs in TRPA1 channels but not in TRPM8 channels.
Supporting Evidence
- TRPA1 activation by various agonists induces Yo-Pro uptake.
- TRPM8 activation does not produce Yo-Pro uptake.
- Pore dilation occurs in TRPA1 but not in TRPM8 channels.
Takeaway
When certain chemicals activate TRPA1, it can change its shape to let bigger particles in, but TRPM8 doesn't do that.
Methodology
The study used HEK293-F cells to test Yo-Pro uptake and ion selectivity changes in TRPA1 and TRPM8 channels.
Limitations
The study does not elucidate the biophysical mechanism underlying pore dilation of TRPA1.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website