Study of the BarA/UvrY System in Shewanella oneidensis MR-1
Author Information
Author(s): Lucas Binnenkade, Jürgen Lassak, Kai M. Thormann
Primary Institution: Max-Planck-Institut für Terrestrische Mikrobiologie, Marburg, Germany
Hypothesis
In S. oneidensis MR-1, the global BarA/UvrY/Csr regulatory pathway is involved in central carbon metabolism processes.
Conclusion
The BarA/UvrY two-component system regulates central carbon metabolism in Shewanella oneidensis MR-1.
Supporting Evidence
- The BarA/UvrY system was shown to regulate the expression of at least two small regulatory RNAs.
- Transcriptomic analysis revealed that UvrY affects the transcript levels of over 200 genes.
- Mutants lacking UvrY showed significant growth differences compared to wild type under specific conditions.
Takeaway
This study shows that a specific system in bacteria helps them manage their food and energy use, especially when they change what they eat.
Methodology
The study used bioinformatic analysis, in vitro phosphotransfer studies, and transcriptomic analysis by microarrays.
Limitations
The study primarily focuses on a single strain and may not represent all Shewanella species.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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