A Communal Bacterial Adhesin Anchors Biofilm and Bystander Cells to Surfaces
2011

How Bap1 and RbmA Help Bacteria Stick Together in Biofilms

publication 10 minutes Evidence: high

Author Information

Author(s): Absalon Cedric, Van Dellen Katrina, Watnick Paula I., Isberg Ralph R.

Primary Institution: Division of Infectious Disease, Children's Hospital, Boston, Massachusetts, United States of America

Hypothesis

The biofilm matrix might harbor proteins with diverse functions.

Conclusion

Bap1 and RbmA play distinct roles in stabilizing biofilm attachment to surfaces and facilitating the recruitment of new bacterial cells.

Supporting Evidence

  • Bap1 is concentrated at the biofilm-surface interface and stabilizes the association of the multilayer biofilm with the surface.
  • RbmA strengthens intercellular attachments and is evenly distributed throughout the biofilm.
  • Exogenous Bap1 increases surface adhesion of planktonic bystanders.

Takeaway

Bacteria can stick together in groups called biofilms, and two special proteins help them attach to surfaces and each other.

Methodology

Proteomic analysis of the V. cholerae biofilm matrix was conducted to identify matrix-associated proteins.

Limitations

The study primarily focuses on two proteins and may not encompass all factors influencing biofilm formation.

Statistical Information

P-Value

0.004

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.ppat.1002210

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication