Simultaneous Detection of Signaling Activity and Gene Expression in Single Cells
Author Information
Author(s): Irene Weibrecht, Ida Nilsson, Mats Söderberg, Ola Soderberg
Primary Institution: Uppsala University
Hypothesis
Can we simultaneously detect individual protein modifications and mRNA molecules in single cells?
Conclusion
The developed method allows for the simultaneous detection of phosphorylated PDGFRβ and DUSP6 mRNA in single cells, revealing cell-to-cell variations.
Supporting Evidence
- The method allows for the detection of individual molecules in fixed cells.
- Cell-to-cell variations in signaling responses were observed.
- The combined method can help in drug screening by pinpointing molecular effects.
Takeaway
The researchers created a new way to see how proteins and genes work together in single cells, helping us understand how cells respond differently to signals.
Methodology
The study combined in situ proximity ligation assay and padlock probes to detect protein modifications and mRNA in single cells.
Limitations
The combined detection method showed a decrease in signal detection efficiency compared to individual methods.
Participant Demographics
TERT immortalized human fibroblast (BJhTert) cells were used.
Digital Object Identifier (DOI)
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