Phosphorylation Sites in Hepatitis C Virus E2 Protein and Interferon Resistance
Author Information
Author(s): Rafique Shazia, Idrees Muhammad, Ilyas Muhammad, Hussain Abrar, Ali Muhammad, Ali Liaqat, Butt Sadia, Afzal Samia, Ur Rehman Irshad, Saleem Sana
Primary Institution: National Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
Hypothesis
The study investigates the role of phosphorylation sites 266 and 267 in the E2 protein of hepatitis C virus in mediating interferon resistance.
Conclusion
The study identifies serine 266 and 267 of the HCV E2 gene as potential phosphorylation sites that may contribute to interferon resistance.
Supporting Evidence
- The study involved cloning and sequencing envelope protein coding genes from local HCV isolates.
- Phylogenetic analysis was performed to compare local sequences with global HCV sequences.
- Potential phosphorylation sites were predicted using online servers.
Takeaway
Scientists looked at parts of a virus that might help it resist treatment, finding two specific spots that could be important.
Methodology
The study used in-silico analysis, sequence alignment, cloning, and structural prediction to investigate phosphorylation sites in the E2 protein.
Digital Object Identifier (DOI)
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