Bone Marrow-Derived Cells are Accepted in the Brain of Mice
Author Information
Author(s): Irene Bergwerf, Nathalie De Vocht, Bart Tambuyzer, Jacob Verschueren, Kristien Reekmans, Jasmijn Daans, Abdelilah Ibrahimi, Viggo Van Tendeloo, Shyama Chatterjee, Herman Goossens, Philippe G. Jorens, Veerle Baekelandt, Dirk Ysebaert, Eric Van Marck, Zwi N. Berneman, Annemie Van Der Linden, Peter Ponsaerts
Primary Institution: University of Antwerp
Hypothesis
Can genetically modified bone marrow-derived stromal cells survive and be tolerated in the central nervous system of immunocompetent mice?
Conclusion
The study shows that reporter gene-modified bone marrow-derived stromal cells are immune-tolerated when implanted in the central nervous system of syngeneic immunocompetent mice.
Supporting Evidence
- BMSC-Luc and BMSC-Luc/eGFP/Pac showed high engraftment capacity in the CNS.
- Real-time bioluminescence imaging confirmed the survival of implanted cells.
- Histological analysis indicated the presence of BMSC at the implantation site.
Takeaway
Scientists found that special cells from bone marrow can be put into the brain of mice without being rejected by the immune system.
Methodology
The study involved cell implantation experiments in the CNS of immunocompetent mice using genetically modified bone marrow-derived stromal cells, followed by bioluminescence imaging and histological analysis.
Potential Biases
Potential bias in the interpretation of immune responses due to the use of specific mouse strains.
Limitations
The study primarily focused on syngeneic models, which may not fully represent allogeneic transplant scenarios.
Participant Demographics
Male ROSA26-L-S-L-Luciferase transgenic mice were used for the experiments.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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