Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis
2011

Cadherin-Mediated Bt Resistance in the Sugarcane Borer

publication 10 minutes Evidence: moderate

Author Information

Author(s): Yang Yunlong, Zhu Yu Cheng, Ottea James, Husseneder Claudia, Leonard B. Rogers, Abel Craig, Luttrell Randall, Huang Fangneng

Primary Institution: Louisiana State University Agricultural Center

Hypothesis

Is the down-regulation of cadherin associated with Cry1Ab resistance in Diatraea saccharalis?

Conclusion

The study found that down-regulation of the cadherin gene DsCAD1 is associated with resistance to Cry1Ab in the sugarcane borer.

Supporting Evidence

  • Cadherin expression was significantly lower in Cry1Ab-resistant strains.
  • RNA interference confirmed the role of cadherin in susceptibility to Cry1Ab.
  • Total alkaline phosphatase activity was similar between susceptible and resistant strains.

Takeaway

The sugarcane borer can resist a type of pesticide because it has less of a certain protein that the pesticide usually binds to.

Methodology

The study involved comparing gene expression levels and conducting RNA interference to assess the role of cadherin in Cry1Ab resistance.

Limitations

The study may not account for other potential resistance mechanisms beyond cadherin expression.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025783

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