Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses
2011
CD99's Role in T Cell Activation and Its Associations
publication
Evidence: moderate
Author Information
Author(s): Pata Supansa, Otáhal Pavel, Brdička Tomáš, Laopajon Witida, Mahasongkram Kodchakorn, Kasinrerk Watchara
Primary Institution: Chiang Mai University, Thailand
Hypothesis
Does CD99 interact with MHC class I, MHC class II, and CD81 to influence T cell activation?
Conclusion
CD99 interacts with MHC class I, MHC class II, and CD81, and its engagement can inhibit T cell proliferation.
Supporting Evidence
- CD99 was shown to associate with MHC class I and II and CD81 on the cell surface.
- Engagement of CD99 with an antibody inhibited T cell proliferation.
- CD99 is recruited into the immunological synapse during T cell activation.
Takeaway
CD99 is a protein on T cells that helps them activate, and it works with other proteins to do this. When CD99 is engaged, it can stop T cells from growing.
Methodology
The study used co-immunoprecipitation and Western blotting to analyze the interactions of CD99 with MHC class I, MHC class II, and CD81 in various cell lines.
Digital Object Identifier (DOI)
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