Two-dimensional cell membrane chromatography guided screening of myocardial protective compounds from Yindan Xinnaotong soft capsule
2025

Screening Heart-Protective Compounds from Yindan Xinnaotong Soft Capsule

publication Evidence: high

Author Information

Author(s): Shao Si-Min, Ji Xuan, Wang Xing, Liu Run-Zhou, Cai Yu-Ru, Lin Xiaobing, Zeng Ze-Jie, Chen Ling, Yang Liu, Yang Hua

Primary Institution: State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University

Hypothesis

Can a two-dimensional cell membrane chromatography system effectively screen for myocardial protective compounds in Yindan Xinnaotong soft capsule?

Conclusion

The study successfully identified 24 potential myocardial protective compounds from Yindan Xinnaotong soft capsule using a novel screening method.

Supporting Evidence

  • 24 potential myocardial protective compounds were identified, including flavonoids, phenolic acids, saponins, and tanshinones.
  • The FOXO signaling pathway was highlighted as a significant mechanism for the cardioprotective effects of Yindan Xinnaotong.
  • All screened compounds showed strong binding affinities with the FOXO3 protein.

Takeaway

Researchers found 24 compounds in a traditional medicine that might help protect the heart, using a special testing method.

Methodology

The study used a two-dimensional H9c2/CMC-RPLC-MS system to screen for active compounds in Yindan Xinnaotong soft capsule.

Digital Object Identifier (DOI)

10.1186/s13020-024-01046-1

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