Cloning and Characterization of a Heat-Shock Protein from Arabian Camels
Author Information
Author(s): Elrobh Mohamed S., Alanazi Mohammad S., Khan Wajahatullah, Abduljaleel Zainularifeen, Al-Amri Abdullah, Bazzi Mohammad D.
Primary Institution: King Saud University
Hypothesis
The study aims to clone and characterize a full-length cDNA encoding a putative stress-induced heat shock protein from the Arabian camel.
Conclusion
The cDNA sequences of the HSPA6 gene and its protein structure from the Arabian camel are highly conserved and similar to those of other mammalian species.
Supporting Evidence
- The study is the first report of molecular cloning and characterization of the HSPA6 protein from the Arabian camel.
- The HSPA6 gene showed high similarity (77–91%) with heat shock genes from other mammals.
- The predicted protein has an estimated molecular weight of 70.5 kDa and an isoelectric point of 6.0.
- The camel HSPA6 protein structure exhibited 87% and 82% similarity with human and mouse HSPs, respectively.
Takeaway
This study found a special protein in camels that helps them survive in hot and dry places, and this protein is very similar to those found in other animals.
Methodology
The study used rapid amplification of cDNA ends (RACE) to clone the full-length cDNA of the HSPA6 gene from camel liver tissues.
Limitations
The study primarily focuses on the molecular cloning and characterization without exploring the functional roles of the HSPA6 protein in detail.
Participant Demographics
Camel liver tissues from freshly sacrificed young adult male camels were used.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website