Getting a Transcription Factor to Only One Nucleus Following Mitosis
2008
Getting a Transcription Factor to Only One Nucleus Following Mitosis
publication
Evidence: moderate
Author Information
Author(s): Emily J. Parnell, David J. Stillman
Primary Institution: University of Utah Health Sciences Center
Hypothesis
How does the Cbk1 kinase regulate the asymmetric localization of the Ace2 transcription factor in daughter cells?
Conclusion
The Cbk1 kinase directs daughter-specific nuclear accumulation of the Ace2 transcription factor by blocking its export from the nucleus.
Supporting Evidence
- Cbk1 is essential for the daughter-specific nuclear localization of Ace2.
- Ace2 accumulates in the daughter cell nucleus due to the action of Cbk1.
- Phosphorylation by Cbk1 inactivates the nuclear export signal of Ace2.
- The study provides direct evidence of how Cbk1 regulates Ace2 localization.
Takeaway
This study shows how a protein called Cbk1 helps another protein, Ace2, stay in the right part of a cell after it divides, which is important for the cell's function.
Methodology
The study used time lapse microscopy and peptide scanning arrays to investigate the localization and phosphorylation of Ace2 and Cbk1.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website