IFIT-2 Reduces Inflammatory Protein Production
Author Information
Author(s): Susanne Berchtold, Birgit Manncke, Juliane Klenk, Julia Geisel, Ingo B Autenrieth, Erwin Bohn
Primary Institution: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Tübingen, Tübingen, Germany
Hypothesis
The study aims to characterize the function of IFIT-2 in regulating protein expression in response to LPS stimulation.
Conclusion
IFIT-2 may selectively inhibit LPS induced protein expression by affecting mRNA stability.
Supporting Evidence
- IFIT-2 inhibits LPS induced expression of TNF-α, IL-6, and MIP-2.
- Forced IFIT-2 expression reduced TNF-α secretion by more than 90%.
- The stability of TNF-α mRNA was significantly decreased in cells overexpressing IFIT-2.
Takeaway
IFIT-2 is a protein that helps control how much of certain inflammatory proteins are made when the body is fighting infections.
Methodology
The study used stably transfected RAW264.7 macrophages to assess the impact of IFIT-2 on protein expression and mRNA stability after LPS stimulation.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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