Nocodazole Treatment Decreases Expression of Pluripotency Markers in Human Embryonic Stem Cells
Author Information
Author(s): Kallas Ade, Pook Martin Maimets, Martti Zimmermann, Külli Maimets, Toivo Maimets
Primary Institution: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Hypothesis
How does nocodazole treatment influence the expression of pluripotency markers in human embryonic stem cells?
Conclusion
Nocodazole treatment leads to a decrease in the expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells.
Supporting Evidence
- Nocodazole treatment resulted in a decrease of cell population positive for all four markers Nanog, Oct4, SSEA-3, and SSEA-4.
- After nocodazole wash-out, the expression of pluripotency markers remained low despite normal cell cycle distribution.
- Nocodazole treatment increased the rate of apoptosis in human embryonic stem cells.
Takeaway
Nocodazole is a drug that stops cells from dividing, and when human embryonic stem cells are treated with it, they lose important markers that keep them in a stem cell state.
Methodology
Flow cytometry was used to analyze the expression of pluripotency markers in human embryonic stem cells treated with nocodazole.
Limitations
The study did not explore the long-term effects of nocodazole treatment on stem cell differentiation.
Participant Demographics
Human embryonic stem cell line WA09 was used in the study.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website