Protochlamydia naegleriophila as Etiologic Agent of Pneumonia
2008

Protochlamydia naegleriophila and Pneumonia

Sample size: 134 publication Evidence: moderate

Author Information

Author(s): Casson Nicola, Michel Rolf, Müller Karl-Dieter, Aubert John David, Greub Gilbert

Primary Institution: University Hospital Center and University of Lausanne

Hypothesis

Is Protochlamydia naegleriophila a new etiologic agent of pneumonia?

Conclusion

The study identified Protochlamydia naegleriophila as a potential new agent of pneumonia in an immunocompromised patient.

Supporting Evidence

  • One patient with pneumonia tested positive for Protochlamydia naegleriophila using the developed PCR.
  • The 16Sr RNA sequence of KNic showed 97.6% similarity with Pr. amoebophila.
  • Immunofluorescence confirmed the presence of Protochlamydia antigen in the bronchoalveolar lavage sample.

Takeaway

Scientists found a new germ that might cause pneumonia, and they created a test to find it in sick people.

Methodology

The study involved growing a Naegleria endosymbiont in ameba coculture, developing a specific PCR diagnostic test, and testing bronchoalveolar lavage samples.

Limitations

Further studies are needed to fully assess the role of Protochlamydia spp. in pneumonia.

Participant Demographics

The positive sample was from an immunocompromised patient with pneumonia symptoms.

Digital Object Identifier (DOI)

10.3201/eid1401.070980

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