NF-κB and Muscle Protein Degradation in Cancer
Author Information
Author(s): Wyke S M, Tisdale M J
Primary Institution: Pharmaceutical Sciences Research Institute, Aston University, Birmingham, UK
Hypothesis
NF-κB mediates proteolysis-inducing factor induced protein degradation and expression of the ubiquitin–proteasome system in skeletal muscle.
Conclusion
The study confirms that NF-κB activation is essential for PIF-induced protein degradation and upregulation of the ubiquitin–proteasome pathway in skeletal muscle.
Supporting Evidence
- Proteolysis-inducing factor (PIF) caused degradation of I-κBα and nuclear accumulation of NF-κB.
- PIF induced significant increases in total protein loss in wild-type myotubes.
- Mutant I-κBα inhibited PIF-induced protein degradation and proteasome expression.
Takeaway
This study shows that a factor from tumors can make muscle proteins break down, and this happens because of a specific protein called NF-κB.
Methodology
Murine C2C12 myoblasts were transfected with plasmids to study the effects of proteolysis-inducing factor on protein degradation and ubiquitin–proteasome pathway expression.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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