How Yeast Adapt to Chemical Stress
Author Information
Author(s): Lelandais Gaëlle, Tanty Véronique, Geneix Colette, Etchebest Catherine, Jacq Claude, Devaux Frédéric
Primary Institution: INSERM UMR S726, Université Paris 7
Hypothesis
How do Saccharomyces cerevisiae and Candida glabrata respond to drug-induced stress at the genetic level?
Conclusion
The study found that while the gene expression responses to drug stress were conserved between the two yeast species, the regulatory networks governing these responses differed significantly.
Supporting Evidence
- Gene expression patterns in response to drugs were remarkably conserved between Saccharomyces cerevisiae and Candida glabrata.
- Despite similar responses, the regulatory networks in the two species differed significantly.
- Experimental assays confirmed differences in the activities of transcription factors ScYap1p and Cgap1p.
Takeaway
This study shows that two types of yeast can react similarly to stress from drugs, but they use different methods to control their genes.
Methodology
The researchers used comparative transcriptomics, a differential clustering algorithm, and promoter analyses to study gene expression in response to benomyl, an antifungal agent.
Digital Object Identifier (DOI)
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