Modeling the TCA Cycle and Glyoxylate Bypass in Mycobacterium tuberculosis
Author Information
Author(s): Vivek Kumar Singh, Indira Ghosh
Primary Institution: Bioinformatics Centre, University of Pune, Pune, India
Hypothesis
Can kinetic modeling of the TCA cycle and glyoxylate bypass in Mycobacterium tuberculosis help identify potential drug targets?
Conclusion
Kinetic modeling can effectively assess potential drug targets that interfere with the glyoxylate bypass in Mycobacterium tuberculosis.
Supporting Evidence
- The model showed that deleting both isocitrate lyase genes eliminates the bacteria from the lungs.
- Inhibition of isocitrate lyases can be a strategy for targeting persistent mycobacteria.
- The study suggests that both isocitrate dehydrogenases need to be inactivated to allow flux through the glyoxylate bypass.
Takeaway
This study uses computer models to understand how to stop a germ that causes tuberculosis by blocking its food sources.
Methodology
Kinetic models of the TCA cycle and glyoxylate bypass were developed and validated using E. coli data.
Limitations
The models are based on several assumptions that may not fully capture the complexity of the biological systems.
Digital Object Identifier (DOI)
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