Spontaneous Apoptosis in Ovarian Carcinomas and p53 Gene Mutation
Author Information
Author(s): Kupryjánczyk J, Dansonka-Mieszkowska A, Szymánska T, Karpínska G, Rembiszewska A, Rusin M, Konopínski R, Kraszewska E, Timorek A, Yandell D W, Stelmachów J
Primary Institution: The Maria Skl′odowska-Curie Memorial Cancer Center and Institute of Oncology
Hypothesis
The study investigates the association between spontaneous apoptosis and p53 gene mutation in ovarian carcinomas.
Conclusion
The study suggests that p53 alterations positively affect spontaneous apoptosis by increasing growth fraction in ovarian carcinomas.
Supporting Evidence
- The apoptotic index ranged from 0.02 to 0.18 with a mean of 0.11.
- The proliferation index ranged from 3% to 90% with a mean of 54%.
- p53 gene mutations were present in 51 out of 71 tumors.
- p53 protein accumulation was found in 46 tumors.
- Diffuse bcl-2 expression was present in 29 tumors.
- The apoptotic index was positively associated with p53 gene mutation.
- Good histological differentiation negatively affected the apoptotic index.
- FIGO stage and p53 protein accumulation influenced overall survival time.
Takeaway
The study found that changes in a gene called p53 can help cancer cells die, which is important for understanding how ovarian cancer grows.
Methodology
The study used TUNEL method for detecting apoptotic cells, single-strand conformation polymorphism for p53 gene variants, and immunohistochemistry for protein expressions.
Participant Demographics
The study included 64 ovarian, 4 tubal, and 3 peritoneal carcinomas.
Statistical Information
P-Value
P = 0.011
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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