In vitro interactions of Candida parapsilosis wild type and lipase deficient mutants with human monocyte derived dendritic cells
2011

Interactions of Candida parapsilosis with Dendritic Cells

publication Evidence: moderate

Author Information

Author(s): Nagy István, Filkor Kata, Németh Tibor, Hamari Zsuzsanna, Vágvölgyi Csaba, Gácser Attila

Primary Institution: Institute for Plant Genomics, Human Biotechnology and Bioenergy, Bay Zoltán Foundation for Applied Research

Hypothesis

The study aims to assess the function of dendritic cells in fighting Candida parapsilosis and to determine the role of Candida-derived lipase in this interaction.

Conclusion

Dendritic cells are activated by exposure to Candida parapsilosis, leading to increased phagocytosis, killing, and proinflammatory protein secretion.

Supporting Evidence

  • Dendritic cells efficiently phagocytosed and killed both wild type and lipase deficient Candida parapsilosis.
  • Phagocytic and fungicidal activities were more potent for lipase deficient strains compared to wild type.
  • Lipase deficient Candida parapsilosis induced higher expression of proinflammatory cytokines in dendritic cells.

Takeaway

The study shows that certain immune cells can eat and kill a type of yeast called Candida parapsilosis, and that a specific part of the yeast helps it avoid being eaten.

Methodology

The study involved co-culturing human monocyte-derived dendritic cells with wild type and lipase deficient strains of Candida parapsilosis to assess phagocytic capacity and cytokine production.

Participant Demographics

Human peripheral blood mononuclear cells from healthy donors were used to derive dendritic cells.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2180-11-122

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