A Conserved Tissue-Specific Homeodomain-Less Isoform of MEIS1 Is Downregulated in Colorectal Cancer
2011

MEIS1D Protein and Colorectal Cancer

Sample size: 12 publication 10 minutes Evidence: moderate

Author Information

Author(s): Crist Richard C., Roth Jacquelyn J., Waldman Scott A., Buchberg Arthur M.

Primary Institution: Thomas Jefferson University

Hypothesis

What is the role of the MEIS1D isoform in colorectal cancer?

Conclusion

The MEIS1D protein is downregulated in the majority of colorectal cancer samples, suggesting it may act as a tumor suppressor.

Supporting Evidence

  • 83% of primary colorectal cancer samples showed reduced MEIS1D expression compared to normal tissue.
  • MEIS1D was detected in human colon mucosa, indicating its relevance in colorectal health.
  • The study identified two novel isoforms of the MEIS1 protein in murine tissues.

Takeaway

Scientists found that a specific protein, MEIS1D, is less present in colon cancer tissues, which might mean it helps prevent cancer.

Methodology

The study involved analyzing murine gastrointestinal tissue samples and human colorectal cancer samples using western blot and RT-PCR.

Potential Biases

Potential bias in sample selection and the limited number of human samples could affect the generalizability of the findings.

Limitations

The study primarily focused on a limited number of human samples and may not represent all colorectal cancer cases.

Participant Demographics

The study included 12 patients with primary colorectal cancer and matched normal mucosa.

Statistical Information

P-Value

0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023665

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