How CRP Regulates Porin Genes in Yersinia pestis
Author Information
Author(s): Gao He, Zhang Yiquan, Yang Lin, Liu Xia, Guo Zhaobiao, Tan Yafang, Han Yanping, Huang Xinxiang, Zhou Dongsheng, Yang Ruifu
Primary Institution: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Hypothesis
The regulation of porin genes and its own gene by CRP in Yersinia pestis is unclear.
Conclusion
Y. pestis CRP-cAMP has no regulatory effect on the ompR-envZ operon, but it stimulates ompC and ompF directly while repressing ompX.
Supporting Evidence
- CRP in Y. pestis does not regulate the ompR-envZ operon.
- CRP directly stimulates the expression of ompC and ompF.
- CRP represses the expression of ompX.
- No transcriptional regulatory association between CRP and its own gene was detected.
Takeaway
This study shows that a protein called CRP in Y. pestis works differently than in E. coli, controlling certain genes directly without affecting others.
Methodology
The study involved creating mutant strains of Y. pestis and analyzing gene expression through various assays including RT-PCR and lacZ fusion.
Limitations
The study did not detect autoregulation of CRP in Y. pestis under the conditions tested.
Digital Object Identifier (DOI)
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