The Putative RNA Helicase HELZ Promotes Cell Proliferation, Translation Initiation and Ribosomal Protein S6 Phosphorylation
2011
HELZ and Its Role in Cell Growth and Protein Translation
publication
Evidence: moderate
Author Information
Author(s): Hasgall Philippe A., Hoogewijs David, Faza Marius B., Panse Vikram G., Wenger Roland H., Camenisch Gieri
Primary Institution: University of Zürich
Hypothesis
HELZ promotes cell proliferation and translation initiation.
Conclusion
HELZ is involved in global protein translation and promotes cell proliferation by regulating ribosomal protein S6 phosphorylation.
Supporting Evidence
- HELZ overexpression activates global protein translation.
- Downregulation of HELZ reduces translational initiation.
- HELZ interacts with the poly(A)-binding protein (PABP).
- HELZ suppression leads to hypophosphorylation of ribosomal protein S6.
Takeaway
HELZ is a protein that helps cells grow and make other proteins. When HELZ is not working well, cells grow slower.
Methodology
The study used luciferase reporter gene constructs, metabolic labeling, and siRNA-mediated suppression to analyze HELZ's role in protein translation.
Limitations
The study did not analyze the role of HELZ in tumor formation assays.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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