Combining Radiation Therapy and CAR-T Cells for Breast Cancer Treatment
Author Information
Author(s): Aniogo Eric, Kujawski Maciej, Awuah Dennis, Cha Seung E., Espinosa Ruby, Hui Susanta, Ghimire Hemendra, Yazaki Paul J., Brown Christine E., Wang Xiuli, Shively John E.
Primary Institution: City of Hope, Duarte, CA, United States
Hypothesis
Prior treatment with Image-Guided Radiation Therapy (IGRT) would increase CAR-T cell tumor infiltration, leading to improved antigen specific expansion of CAR-T cells.
Conclusion
The combination of IGRT and anti-CEA scFab CAR-T therapy effectively targets both primary tumors and distant metastases in triple-negative breast cancer.
Supporting Evidence
- The anti-CEA scFab CAR-T cells showed robust in vitro expression and lysis of CEA-positive TNBC cells.
- Significantly higher IFN-γ production was observed in CD28z-CAR-T cells compared to 4-1BBz-CAR-T cells.
- Combined IGRT and CAR-T therapy resulted in significant tumor growth reduction compared to either therapy alone.
Takeaway
This study shows that using radiation therapy before CAR-T cell treatment helps the CAR-T cells work better against breast cancer.
Methodology
The study involved engineering anti-CEA CAR-T cells and testing their efficacy in vitro and in vivo using a metastatic triple negative breast cancer model.
Limitations
The study was limited to CAR-T therapy in immunocompromised animals, restricting the ability to assess potential toxicity.
Participant Demographics
The study used T cells from healthy human donors.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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