High levels of the MDM2 oncogene in paediatric rhabdomyosarcoma cell lines may confer multidrug resistance
2001
MDM2 and Multidrug Resistance in Rhabdomyosarcoma
Sample size: 4
publication
Evidence: moderate
Author Information
Author(s): Cocker H A, Hobbs S M, Tiffin N, Pritchard-Jones K, Pinkerton C R, Kelland L R
Primary Institution: The Institute of Cancer Research
Hypothesis
Does the MDM2 oncogene influence sensitivity to chemotherapeutic drugs in rhabdomyosarcoma?
Conclusion
MDM2 overexpression may regulate multidrug resistance in some rhabdomyosarcomas.
Supporting Evidence
- Transfection with the mdm2 gene resulted in increased levels of the MDM2 protein in all cell lines.
- In two cell lines, the mdm2 gene caused a significant increase in resistance to certain chemotherapy drugs.
- The resistance was reversible using the MDR modulator PSC833.
Takeaway
Some cancer cells can become resistant to medicine, and this study found that a specific gene called MDM2 might be a reason why.
Methodology
Stable transfection of the mdm2 gene into 4 human paediatric rhabdomyosarcoma cell lines was performed.
Participant Demographics
Human paediatric rhabdomyosarcoma cell lines.
Digital Object Identifier (DOI)
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