Expression analysis of asthma candidate genes during human and murine lung development
2011

Study of Asthma Genes in Lung Development

Sample size: 38 publication 10 minutes Evidence: moderate

Author Information

Author(s): Melén Erik, Kho Alvin T, Sharma Sunita, Gaedigk Roger, Leeder J Steven, Mariani Thomas J, Carey Vincent J, Weiss Scott T, Tantisira Kelan G

Primary Institution: Channing Laboratory, Brigham and Women's Hospital and Harvard Medical School

Hypothesis

Genes influencing normal airways development would be over-represented by genes associated with asthma.

Conclusion

The study suggests that while asthma susceptibility genes are not generally over-represented during lung development, some genes show consistent differential expression, indicating their potential role in lung development.

Supporting Evidence

  • 96 asthma genes were identified through literature review.
  • Some asthma genes showed consistent differential expression in both human and murine lung development.
  • Genes like NOD1, EDN1, CCL5, RORA, and HLA-G were highlighted for their roles in lung development.

Takeaway

The researchers looked at genes linked to asthma and found that some of them are important for lung development, even though not all asthma genes are more common during this process.

Methodology

The study analyzed gene expression patterns in human lung tissues and three mouse strains during different stages of lung development.

Potential Biases

Potential null bias due to missing recent asthma genes and limitations in the microarray platforms used.

Limitations

The study was limited by a small sample size and the lack of data on key exposures that could influence gene expression.

Participant Demographics

Human fetal lung tissues from 38 subjects with estimated gestational ages of 7-22 weeks.

Statistical Information

P-Value

p<0.001

Confidence Interval

CI 0.90-1.62

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1465-9921-12-86

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