Simplifying Mutagenesis of Herpes Simplex Virus Using Lambda Phage Recombination
Author Information
Author(s): Falko Schmeisser, Jerry P. Weir
Primary Institution: Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
Hypothesis
Can the incorporation of a lambda phage recombination system simplify the mutagenesis of herpes simplex virus bacterial artificial chromosomes?
Conclusion
The improvements to the techniques of HSV BAC mutagenesis will facilitate the construction of recombinant herpes simplex viruses and viral vectors.
Supporting Evidence
- The incorporation of a lambda phage recombination system allows for efficient insertion of DNA fragments.
- Using EGFP helps in selecting the desired recombinant BACs during the mutagenesis process.
- The new method simplifies the construction of allele replacement vectors.
Takeaway
This study found a way to make it easier to change the herpes virus DNA by using a special system that helps insert new pieces of DNA.
Methodology
The study involved the incorporation of a lambda phage recombination system into an allele replacement vector and the use of EGFP for selection of recombinant BACs.
Limitations
The study does not specify the limitations of the new method compared to existing techniques.
Digital Object Identifier (DOI)
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