Identifying Genes That Affect the Cell Cycle in Yeast
Author Information
Author(s): Niu Wei, Li Zhihua, Zhan Wenjing, Iyer Vishwanath R., Marcotte Edward M.
Primary Institution: University of Texas, Austin, Texas, United States of America
Hypothesis
Can we identify new cell cycle regulatory genes through a systematic overexpression screen in yeast?
Conclusion
The study identified 108 genes whose overexpression significantly delays the yeast cell cycle, many of which are newly implicated in cell cycle regulation.
Supporting Evidence
- 108 genes were identified that cause significant delays in the cell cycle when overexpressed.
- Many of the identified genes are newly implicated in cell cycle regulation.
- Flow cytometry was used to quantitatively analyze the effects of gene overexpression.
Takeaway
Researchers looked at many yeast genes to see which ones, when made too active, caused problems in the cell cycle. They found 108 genes that can mess up how yeast cells grow and divide.
Methodology
The study used high-throughput flow cytometry to analyze the effects of overexpression of 5,556 yeast ORFs on cell cycle progression.
Potential Biases
Potential biases may arise from the overexpression system, which could lead to non-physiological effects.
Limitations
The study may not cover all potential cell cycle regulators due to the limitations of the overexpression approach and the yeast model system.
Participant Demographics
The study focused on the yeast species Saccharomyces cerevisiae.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website