Reassessment of CXCR4 Chemokine Receptor Expression in Human Normal and Neoplastic Tissues Using the Novel Rabbit Monoclonal Antibody UMB-2
2008

Study of CXCR4 Chemokine Receptor in Human Tissues

Sample size: 247 publication Evidence: high

Author Information

Author(s): Fischer Thomas, Nagel Falko, Jacobs Stefan, Stumm Ralf, Schulz Stefan

Primary Institution: Julius-Maximilians-University, Würzburg, Germany

Hypothesis

The novel rabbit monoclonal antibody UMB-2 can effectively detect CXCR4 receptors in human normal and neoplastic tissues.

Conclusion

The rabbit monoclonal antibody UMB-2 is effective for assessing CXCR4 receptor status in various human tumors.

Supporting Evidence

  • UMB-2 specifically detects CXCR4 receptors in fixed human tissues.
  • UMB-2 shows effective plasma membrane staining in tumor cells.
  • Previous antibodies failed to adequately detect CXCR4 in human tissues.

Takeaway

Scientists created a special antibody that helps find a specific protein in cancer cells, which can help doctors understand how to treat the cancer better.

Methodology

The study involved immunohistochemical staining of various human tumor samples with the UMB-2 antibody to assess CXCR4 expression.

Limitations

The study primarily focused on the effectiveness of the UMB-2 antibody and did not explore the functional implications of CXCR4 expression in detail.

Digital Object Identifier (DOI)

10.1371/journal.pone.0004069

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