The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules
2006
The gcvB Gene in Yersinia pestis and Its Role in Gene Regulation
publication
Evidence: moderate
Author Information
Author(s): Sarah D McArthur, Sarah C Pulvermacher, George V Stauffer
Primary Institution: The University of Iowa
Hypothesis
The gcvB gene in Yersinia pestis encodes small regulatory RNAs that influence gene expression.
Conclusion
The Y. pestis gcvB gene encodes two small non-coding regulatory RNAs that repress dppA expression, and its deletion affects growth and colony morphology.
Supporting Evidence
- The gcvB gene is activated by the GcvA protein and repressed by the GcvR protein.
- Deletion of the gcvB gene results in a slower growth rate and altered colony morphology.
- The gcvB-encoded RNAs are required for repression of the Y. pestis dppA gene.
Takeaway
The gcvB gene in Y. pestis helps control how the bacteria grow and change shape by regulating other genes.
Methodology
The study involved constructing plasmid fusions, β-galactosidase assays, and Northern blotting to analyze gene expression.
Digital Object Identifier (DOI)
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