Diversity of Citrus tristeza virus in a single host
Author Information
Author(s): Weng Ziming, Barthelson Roger, Gowda Siddarame, Hilf Mark E., Dawson William O., Galbraith David W., Xiong Zhongguo
Primary Institution: University of Arizona
Hypothesis
Can multiple genotypes of an RNA virus within a single host generate extensive genetic diversity?
Conclusion
The study found that persistent infection by multiple genotypes of Citrus tristeza virus leads to significant genetic diversity through recombination.
Supporting Evidence
- Multiple genotypes of Citrus tristeza virus were identified in a single host plant.
- Promiscuous recombination between these genotypes led to a large number of genetic variants.
- Some recombinants showed subsequent divergence, increasing genotypic complexity.
Takeaway
When a plant gets infected by different types of a virus, they can mix together and create many new versions of the virus, making it very diverse.
Methodology
The researchers used a resequencing microarray and deep sequencing to analyze the genetic complexity of Citrus tristeza virus in a persistent infection.
Limitations
The study may not account for all minor genotypes present in the viral population.
Digital Object Identifier (DOI)
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