New Model for Testing Burn Wound Treatments
Author Information
Author(s): Chen Ping, Sebastian Eliza A., Karna S. L. Rajasekhar, Leung Kai P.
Primary Institution: United States Army Institute of Surgical Research
Hypothesis
Can a stringent ex vivo-burned porcine skin model provide high in vivo relevance for screening topical antimicrobials?
Conclusion
The ex vivo model developed in our lab is a stringent and effective platform for antimicrobial activity screening, showing high relevance to in vivo outcomes.
Supporting Evidence
- Approximately 1% of the commensals survived the skin burn, affecting treatment outcomes.
- Silvadene and Flammacerium showed ~1–3 log reduction in viable counts against six pathogens.
- The ex vivo treatment outcomes of Silvadene and Flammacerium were comparable to in vivo results.
Takeaway
Researchers created a new way to test burn treatments using pig skin that better mimics real-life infections, helping find better medicines.
Methodology
A 3 cm-diameter thermal injury was created on porcine skin, inoculated with pathogens, and treated with various antimicrobial agents to evaluate efficacy.
Limitations
The model lacks blood circulation and an immune system, and does not represent chronic wounds or biofilm-mediated infections.
Digital Object Identifier (DOI)
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