High Levels of ACC2 in Human Adipose Tissue and a Novel Isoform
Author Information
Author(s): Castle John C., Hara Yoshikazu, Raymond Christopher K., Garrett-Engele Philip, Ohwaki Kenji, Kan Zhengyan, Kusunoki Jun, Johnson Jason M.
Primary Institution: Rosetta Inpharmatics LLC, Seattle, Washington, United States of America
Hypothesis
ACC2 is expressed at higher levels in human white adipose tissue compared to rodent models.
Conclusion
The study found that human adipose tissue expresses more ACC2 than ACC1, and identified a novel isoform of ACC2 with enzymatic activity.
Supporting Evidence
- Human adipose tissue expresses more ACC2 mRNA than ACC1.
- A novel isoform of ACC2 (ACC2.v2) was identified in human adipose tissue.
- ACC2.v2 has enzymatic activity and is endogenously expressed in adipose.
- ACC2 expression patterns differ significantly between humans and rodents.
Takeaway
This study shows that human fat has a lot of a protein called ACC2, which helps make fat, and there's a new version of this protein that works differently.
Methodology
The researchers used RT-PCR, real-time PCR, and immunoprecipitation to analyze ACC2 expression in human adipose tissue.
Limitations
The study acknowledges potential variations in tissue dissection and RNA isolation methods that could affect results.
Digital Object Identifier (DOI)
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