How HPV16 Enters Cells Without Clathrin or Caveolin
Author Information
Author(s): Spoden Gilles, Freitag Kirsten, Husmann Matthias, Boller Klaus, Sapp Martin, Lambert Carsten, Florin Luise
Primary Institution: Institute for Medical Microbiology and Hygiene, University of Mainz, Mainz, Germany
Hypothesis
Does HPV16 enter host cells through clathrin- and caveolin-independent mechanisms involving tetraspanin-enriched microdomains?
Conclusion
HPV16 enters epithelial cells via tetraspanin-enriched microdomains, independent of clathrin and caveolin pathways.
Supporting Evidence
- HPV16 entry was shown to be independent of clathrin and caveolin pathways.
- Tetraspanin-specific antibodies inhibited HPV16 cell entry and infection.
- Virions associated with tetraspanin-enriched microdomains during infection.
Takeaway
HPV16 can sneak into cells without using the usual pathways that other viruses use, by hitching a ride on special areas of the cell membrane called tetraspanin-enriched microdomains.
Methodology
The study used immunofluorescence and infection assays to analyze HPV16 entry mechanisms in HeLa cells.
Digital Object Identifier (DOI)
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