Finding Inhibitors for Pseudomonas aeruginosa Exoenzyme S Using Yeast
Author Information
Author(s): Arnoldo Anthony, Curak Jasna, Kittanakom Saranya, Chevelev Igor, Lee Vincent T., Sahebol-Amri Mehdi, Koscik Becky, Ljuma Lana, Roy Peter J., Bedalov Antonio, Giaever Guri, Nislow Corey, Merrill Rod A., Lory Stephen, Stagljar Igor
Primary Institution: University of Toronto
Hypothesis
Bacterial virulence proteins could act similarly in both yeast and human cells.
Conclusion
The study identified exosin as the first small molecule inhibitor of Exoenzyme S, which protects against Pseudomonas infection.
Supporting Evidence
- Exosin was shown to directly interact with ExoS in vitro as a competitive inhibitor.
- Exosin protects CHO cells from lysis during Pseudomonas aeruginosa infection.
- Yeast homologues of human ExoS targets were identified, validating the yeast model.
- Six potential inhibitors were identified from a library of over 56,000 compounds.
Takeaway
Researchers used yeast to find a new medicine that can help fight a harmful germ called Pseudomonas aeruginosa.
Methodology
A yeast phenotypic assay combined with a large-scale inhibitor screen was used to identify small molecule inhibitors.
Limitations
The study primarily focused on a single bacterial toxin and its inhibitors.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website