How Dendritic Cells and Monocytes Interact During Listeria Infection
Author Information
Author(s): Dilnawaz Kapadia, Aida Sadikovic, Yannick Vanloubbeeck, Dirk Brockstedt, Lawrence Fong
Primary Institution: University of California San Francisco
Hypothesis
Different antigen presenting cells (APCs) could make varying contributions to induction of Lm-specific immunity.
Conclusion
Monocytes enhance cytokine production during Listeria infection but inhibit T cell priming.
Supporting Evidence
- CD8α+ dendritic cells are the most susceptible to Listeria infection.
- Monocytes secrete high levels of TNFα and IL-12 p70 during infection.
- Co-culture of infected dendritic cells and monocytes enhances cytokine production.
- Infected monocytes inhibit T cell priming against Listeria-derived antigens.
Takeaway
This study shows that while some immune cells help fight infections, others can actually make it harder for the body to respond properly.
Methodology
The study involved isolating primary antigen presenting cells from mice, infecting them with Listeria, and analyzing their interactions and cytokine production.
Limitations
The study primarily used a mouse model, which may not fully represent human immune responses.
Participant Demographics
Mice used were wild-type C57BL/6 and transgenic OT1 TCR mice.
Digital Object Identifier (DOI)
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