Cloning and Characterization of Porcine 4Ig-B7-H3: A Potent Inhibitor of Porcine T-Cell Activation
Author Information
Author(s): Chen Weiwei, Hou Zhibo, Li Chunman, Xiong Sheng, Liu Henggui
Primary Institution: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China
Hypothesis
It is not known whether a counterpart of human or murine B7-H3 exists in porcine species.
Conclusion
The porcine 4Ig-B7-H3 serves as a negative regulator for the T-cell immune response.
Supporting Evidence
- Porcine 4Ig-B7-H3 was found to inhibit T cell proliferation and cytokine production.
- The protein encoded by the porcine 4ig-b7-h3 gene was 90.88% identical to human 4Ig-B7-H3.
- Porcine B7-H3 was broadly distributed in various porcine tissues.
- Two isoforms of B7-H3 were identified in porcine tissues, with 4Ig-B7-H3 being the dominant form.
Takeaway
Scientists found a new protein in pigs that stops their immune cells from working too hard, which is similar to a protein found in humans.
Methodology
The porcine 4ig-b7-h3 gene was cloned using RT-PCR and 3′-terminus RACE, and its protein sequence was analyzed for homology with human B7-H3.
Limitations
The study did not identify the receptor for porcine B7-H3.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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