Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
2008

VEGF's Role in Cervical Remodeling in Pregnant Rodents

Sample size: 20 publication Evidence: moderate

Author Information

Author(s): Mowa Chishimba, Li Tianbo, Jesmin Subrina, Folkesson Hans G, Usip Sharon E, Papka Raymond E, Hou Guichuan

Primary Institution: Appalachian State University

Hypothesis

Factors that regulate the cervical vasculature, particularly VEGF, play a critical role in cervical remodeling.

Conclusion

VEGF regulates key factors involved in cervical remodeling and induces cervical epithelial growth.

Supporting Evidence

  • About 4,200 genes were altered in expression by VEGF.
  • VEGF elevated VCAM-1 levels by about 10 folds.
  • VEGF agents altered mRNA levels of decorin, eNO, PLC, and PKC.

Takeaway

VEGF helps the cervix change during pregnancy, which is important for childbirth.

Methodology

DNA microarray analysis, Real-time PCR, and SEM were used to analyze gene expression and cervical morphology.

Limitations

The study only shows alterations in mRNA levels and not protein.

Participant Demographics

Timed-pregnant Sprague Dawley rats and pregnant mice.

Statistical Information

P-Value

<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1477-7827-6-64

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