Vitronectin's Role in Apoptotic and Necrotic Cell Binding
Author Information
Author(s): Stepanek Ondrej, Brdicka Tomas, Angelisova Pavla, Horvath Ondrej, Spicka Jiri, Stockbauer Petr, Man Petr, Horejsi Vaclav
Primary Institution: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic
Hypothesis
Does vitronectin bind to late apoptotic and necrotic cells, and if so, how does this interaction occur?
Conclusion
Vitronectin binds to late apoptotic and necrotic cells, potentially serving as a marker for membrane disruption.
Supporting Evidence
- Vitronectin was identified as the antigen recognized by the 2E12 antibody.
- Flow cytometry showed that vitronectin binds to late apoptotic and necrotic cells.
- Confocal microscopy revealed that vitronectin binds to intracellular structures in late apoptotic cells.
Takeaway
Vitronectin is a protein that helps identify dead cells, which can be important for understanding how our body heals after injury.
Methodology
The study used flow cytometry and confocal microscopy to analyze the binding of vitronectin to late apoptotic and necrotic cells.
Digital Object Identifier (DOI)
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