Optimizing Collagen Extraction from Fish Scales
Author Information
Author(s): Makgobole Mokgadi Ursula, Onwubu Stanley Chibuzor, Baruwa Abayomi, Mpofana Nomakhosi, Obiechefu Zodidi, Naidoo Deneshree, Khathi Andile, Mkhwanazi Blessing
Primary Institution: Durban University of Technology
Hypothesis
The study aims to optimize the extraction of collagen from fish scales using Tris-Glycine buffer through the Taguchi method.
Conclusion
The study found that optimal conditions for collagen extraction yield a purity of 17.14 mg/g from fish scales.
Supporting Evidence
- The optimal conditions for collagen extraction were identified as 0.5 M acetic acid, 100 mL volume, 120 min soaking time, and 10 mL Tris-Glycine buffer.
- FTIR analysis confirmed the successful extraction of type I collagen.
- SDS-PAGE analysis showed a protein banding pattern consistent with collagen.
- The study achieved a collagen yield of 17.14 ± 0.05 mg/g of fish scales.
- R2 value of the mathematical model was 73.84%, indicating a good fit.
- Significant factors affecting collagen yield included acetic acid concentration and buffer volume.
- Further experimental validation is needed to confirm the optimized conditions.
- The study contributes to sustainable practices by utilizing fish scales, a byproduct of the seafood industry.
Takeaway
This study shows how to get collagen from fish scales, which is good for making products like medicine and cosmetics, using a special method to make it easier and better.
Methodology
The Taguchi method was used to optimize extraction parameters including acetic acid concentration, volume, soaking time, and buffer volume.
Limitations
The study did not perform experimental validation of the optimized process.
Statistical Information
P-Value
0.000
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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