Amplifying CD8+ Suppressor T Cells in Mice
Author Information
Author(s): Sharafieh Roshanak, Lemire Yen Powell, Sabrina O'Rourke, James Cone, Robert E.
Primary Institution: University of Connecticut Health Center
Hypothesis
The number and/or activity of CD8+ suppressor T cells should be influenced by an immune response to the antigen.
Conclusion
The study demonstrates that immunization amplifies the numbers of antigen-specific splenic CD8+ suppressor T cells.
Supporting Evidence
- The number of AC-SPL cells required for a 50% reduction in swelling is reduced by injecting a cell population enriched for CD8+ AC-SPL cells.
- Immunizing the mice receiving intracameral antigen to the same antigen decreases the RSw50 of AC-SPL cells required to inhibit the expression of DTH.
- The suppression of DTH is proportional to the number of AC-SPL cells injected.
Takeaway
When mice are given a specific antigen, their immune system makes special cells that help control other immune responses, and this study shows how those cells can be increased.
Methodology
Mice were immunized with an antigen injected into the anterior chamber of the eye, and spleen cells were analyzed for their ability to suppress delayed-type hypersensitivity.
Limitations
The suppressive activity of the CD8+ T cells was transient and only effective for a limited time after injection.
Participant Demographics
Female or male BALB/c or C57Bl/6 mice, 8–10 weeks old.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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