Detection of Human Cytomegalovirus in Sperm and Testis Culture
Author Information
Author(s): Naumenko Victor A, Tyulenev Yurii A, Yakovenko Sergei A, Kurilo Lubov' F, Shileyko Ludmila V, Segal Aleksander S, Zavalishina Larisa E, Klimova Regina R, Tsibizov Anton S, Alkhovskii Sergei V, Kushch Alla A
Primary Institution: The D. I. Ivanovsky Institute of Virology, Ministry of Health and Social Development of the Russian Federation
Hypothesis
Can human cytomegalovirus (HCMV) localize in male germ cells and affect spermatogenesis?
Conclusion
HCMV was found in male germ cells, indicating it can infect immature germ cells and potentially contribute to male infertility.
Supporting Evidence
- HCMV DNA was detected in 12.3% of motile spermatozoa samples.
- Infectious activity was found in 2.9% of samples without significant differences between infertile and fertile men.
- On day 14 of culture, a significant decrease in immature germ cells was observed in HCMV-infected cultures.
Takeaway
This study found a virus in sperm that might make it harder for men to have babies.
Methodology
Semen samples were analyzed using real-time PCR, rapid culture method, and testis organotypic culture.
Potential Biases
Potential bias in sample selection as only men attending an infertility clinic were included.
Limitations
The study may not fully represent all male infertility cases due to the specific sample population.
Participant Demographics
138 men, including 91 infertile and 47 fertile men.
Statistical Information
P-Value
p<0.05
Confidence Interval
0.25%-15%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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