Differential regulation of specific genes in MCF-7 and the ICI 182780-resistant cell line MCF-7/182R-6
1999
Study of Gene Regulation in Breast Cancer Cells
publication
Author Information
Author(s): Jensen B L, Skouv J, Lundholt B K, Lykkesfeldt A E
Primary Institution: Department of Tumor Endocrinology, Institute of Cancer Biology, Copenhagen Ø, Denmark
Hypothesis
What are the mechanisms involved in anti-oestrogen resistance in breast cancer cells?
Conclusion
The study found that the ICI 182780-resistant breast cancer cell line has evolved to grow independently of oestradiol.
Supporting Evidence
- MCF-7/182R-6 cells express a reduced level of estrogen receptor protein.
- The estrogen receptor protein is functional in binding to estrogen and anti-estrogens.
- The resistant cell line has evolved to an estrogen-independent growth phenotype.
Takeaway
Researchers looked at how certain breast cancer cells resist treatment and found that some can grow without the hormone they usually need.
Methodology
The study compared two human breast cancer cell lines regarding their estrogen receptor expression and function.
Digital Object Identifier (DOI)
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