Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice
2008

Stem Cell-Derived Vascular Cells Help Heal Stroke Damage in Mice

Sample size: 12 publication 10 minutes Evidence: high

Author Information

Author(s): Oyamada Naofumi, Itoh Hiroshi, Sone Masakatsu, Yamahara Kenichi, Miyashita Kazutoshi, Park Kwijun, Taura Daisuke, Inuzuka Megumi, Sonoyama Takuhiro, Tsujimoto Hirokazu, Fukunaga Yasutomo, Tamura Naohisa, Nakao Kazuwa

Primary Institution: Kyoto University Graduate School of Medicine

Hypothesis

Can vascular cells derived from human embryonic stem cells contribute to vascular regeneration and therapeutic benefit for the ischemic brain after stroke?

Conclusion

Transplantation of endothelial and mural cells derived from human embryonic stem cells can significantly improve vascular regeneration and reduce infarct area after stroke.

Supporting Evidence

  • Transplanted endothelial cells were incorporated into host capillaries.
  • Significant improvement in cerebral blood flow and vascular density was observed.
  • Reduction of infarct volume and apoptosis was noted in cell mixture-transplanted mice.

Takeaway

Scientists found that using special cells from human embryos can help heal the brain after a stroke in mice by making new blood vessels.

Methodology

Mice underwent middle cerebral artery occlusion and received transplants of endothelial and mural cells derived from human embryonic stem cells.

Potential Biases

Potential bias in the selection of cell types and methods of assessment.

Limitations

The study was conducted in mice, and results may not directly translate to humans.

Participant Demographics

Adult male C57 BL6/J mice weighing 20–25 g.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1479-5876-6-54

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