Extracting Functional Proteins from Inclusion Bodies
Author Information
Author(s): Špela Peternel, Joze Grdadolnik, Vladka Gaberc-Porekar, Radovan Komel
Primary Institution: National Institute of Chemistry, Ljubljana, Slovenia
Hypothesis
Can biologically active proteins be extracted from inclusion bodies without renaturation?
Conclusion
The study demonstrates that a significant amount of biologically active proteins can be extracted from inclusion bodies without renaturation, which is important for the biopharmaceutical industry.
Supporting Evidence
- Proteins in inclusion bodies can retain biological activity when extracted under mild conditions.
- Low temperature during protein production increases the amount of correctly folded proteins in inclusion bodies.
- Significant amounts of G-CSF and GFP can be extracted from inclusion bodies without renaturation.
Takeaway
This study shows that proteins can be trapped in clumps called inclusion bodies, but we can still get them out and use them without messing them up.
Methodology
The study involved producing proteins in E. coli, isolating inclusion bodies, and extracting proteins using mild detergents under non-denaturing conditions.
Limitations
The study primarily focuses on specific proteins and may not generalize to all proteins or conditions.
Digital Object Identifier (DOI)
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