Whole-genome linkage analysis in mapping alcoholism genes using single-nucleotide polymorphisms and microsatellites
2005

Mapping Alcoholism Genes Using SNPs and Microsatellites

Sample size: 1614 publication 10 minutes Evidence: moderate

Author Information

Author(s): Wang Shuang, Huang Song, Liu Nianjun, Chen Liang, Oh Cheongeun, Zhao Hongyu

Primary Institution: Columbia University

Hypothesis

How do whole-genome scans using SNPs compare to those using microsatellites in identifying genes associated with alcoholism?

Conclusion

The study found that SNP scans provided stronger linkage signals for alcoholism than microsatellite scans.

Supporting Evidence

  • Both SNP and microsatellite scans indicated strong linkage signals on chromosomes 2 and 7.
  • The SNP scan provided stronger linkage signals than the microsatellite scan.
  • Three regions of significant linkages were detected in the SNP scan but not in the microsatellite scan.
  • The higher information content of SNPs is a major advantage over microsatellites.

Takeaway

Researchers looked at DNA from many families to find genes linked to alcoholism, and they found that a newer method using SNPs worked better than an older method using microsatellites.

Methodology

Nonparametric linkage analysis using SNPs and microsatellites on 143 pedigrees.

Potential Biases

Potential genotyping errors were addressed, but their impact was small.

Limitations

The study's findings may not be generalizable due to the specific dataset used.

Participant Demographics

143 pedigrees with 1,614 individuals genotyped.

Statistical Information

P-Value

0.0012

Confidence Interval

1-LOD intervals ranged from 9 cM to 40 cM.

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2156-6-S1-S28

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