Meiotic Recombination Hotspots of Fission Yeast Are Directed to Loci that Express Non-Coding RNA
2008
Meiotic Recombination Hotspots in Fission Yeast and Non-Coding RNA
Sample size: 68
publication
Evidence: high
Author Information
Author(s): Wahls Wayne P., Siegel Eric R., Davidson Mari K.
Primary Institution: University of Arkansas for Medical Sciences
Hypothesis
Are meiotic double-strand break hotspots directed to loci that express non-coding RNA?
Conclusion
Meiotic double-strand break hotspots are directed to loci that express polyadenylated non-coding RNAs, suggesting a mechanism for positioning recombination.
Supporting Evidence
- About 56% of the representative non-coding RNA loci are associated with double-strand break peaks.
- 18 out of 68 non-coding RNA molecules map entirely within prominent double-strand break peaks.
- The probability of observing 24 or more occurrences of non-coding RNA within double-strand break peaks is low.
Takeaway
This study found that certain RNA molecules help guide where DNA breaks happen during the formation of eggs and sperm in yeast.
Methodology
The study compared the genome-wide distribution of double-strand break peaks to that of polyadenylated non-coding RNA molecules.
Statistical Information
P-Value
p=2.5×10−8
Confidence Interval
96.4%
Statistical Significance
p≤5.5×10−8
Digital Object Identifier (DOI)
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