Buxus sempervirens Extract Kills Breast Cancer Cells
Author Information
Author(s): Ait-Mohamed Ouardia, Battisti Valentine, Joliot Véronique, Fritsch Lauriane, Pontis Julien, Medjkane Souhila, Redeuilh Catherine, Lamouri Aazdine, Fahy Christine, Rholam Mohamed, Atmani Djebbar, Ait-Si-Ali Slimane
Primary Institution: Laboratoire de Biochimie Appliquée, Université de Béjaia, Algeria
Hypothesis
Does the acetonic extract of Buxus sempervirens induce cell cycle arrest, apoptosis, and autophagy in breast cancer cells?
Conclusion
The Buxus sempervirens extract shows promising anti-cancer activity by inducing both autophagic cell death and apoptosis in breast cancer cells.
Supporting Evidence
- The extract showed cytotoxic activity towards all five breast cancer cell lines tested.
- IC50 values ranged from 7.74 µg/ml to 12.5 µg/ml for cancer cells and 19.24 µg/ml for the control cell line.
- Cell cycle arrest was observed in the G0/G1 phase in treated cancer cells.
- Autophagy was confirmed by the presence of autophagic markers in treated cells.
- Downregulation of cyclin D1 was noted in cancer cells after treatment.
Takeaway
A plant extract from Buxus sempervirens can help kill breast cancer cells by making them stop growing and die in two different ways.
Methodology
The study used MTT assays to evaluate cytotoxicity, FACS analysis for cell cycle and apoptosis assessment, and immunoblotting for protein expression analysis.
Limitations
The study primarily focuses on in vitro results, which may not fully represent in vivo effects.
Digital Object Identifier (DOI)
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